Antibody data
- Antibody Data
- Antigen structure
- References [20]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
Reference
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- Product number
- 14477-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#14477-1-AP, RRID:AB_2288297
- Product name
- VAPB antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated VAPB antibody (Cat. #14477-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Serine palmitoyltransferase assembles at ER-mitochondria contact sites.
Mobile late endosomes modulate peripheral endoplasmic reticulum network architecture.
The role of Mfn2 in the structure and function of endoplasmic reticulum-mitochondrial tethering in vivo.
The Novel ALG-2 Target Protein CDIP1 Promotes Cell Death by Interacting with ESCRT-I and VAPA/B.
VPS13D interacts with VCP/p97 and negatively regulates endoplasmic reticulum-mitochondria interactions.
A High-Density Human Mitochondrial Proximity Interaction Network.
Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation.
Nir2 Is an Effector of VAPs Necessary for Efficient Hepatitis C Virus Replication and Phosphatidylinositol 4-Phosphate Enrichment at the Viral Replication Organelle.
A trap mutant reveals the physiological client spectrum of TRC40.
MIGA2 Links Mitochondria, the ER, and Lipid Droplets and Promotes De Novo Lipogenesis in Adipocytes.
Long-chain acyl-CoA synthetase 1 interacts with key proteins that activate and direct fatty acids into niche hepatic pathways.
Loss of VAPB Regulates Autophagy in a Beclin 1-Dependent Manner.
DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.
Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR.
Adenovirus Modulates Toll-Like Receptor 4 Signaling by Reprogramming ORP1L-VAP Protein Contacts for Cholesterol Transport from Endosomes to the Endoplasmic Reticulum.
The ATF6 pathway of the ER stress response contributes to enhanced viability in glioblastoma.
The role of endoplasmic reticulum-related BiP/GRP78 in interferon gamma-induced persistent Chlamydia pneumoniae infection.
Expression of vesicle-associated membrane-protein-associated protein B cleavage products in peripheral blood leukocytes and cerebrospinal fluid of patients with sporadic amyotrophic lateral sclerosis.
Widespread aggregation of mutant VAPB associated with ALS does not cause motor neuron degeneration or modulate mutant SOD1 aggregation and toxicity in mice.
Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation.
Aaltonen MJ, Alecu I, König T, Bennett SA, Shoubridge EA
Life science alliance 2022 Feb;5(2)
Life science alliance 2022 Feb;5(2)
Mobile late endosomes modulate peripheral endoplasmic reticulum network architecture.
Spits M, Heesterbeek IT, Voortman LM, Akkermans JJ, Wijdeven RH, Cabukusta B, Neefjes J
EMBO reports 2021 Mar 3;22(3):e50815
EMBO reports 2021 Mar 3;22(3):e50815
The role of Mfn2 in the structure and function of endoplasmic reticulum-mitochondrial tethering in vivo.
Han S, Zhao F, Hsia J, Ma X, Liu Y, Torres S, Fujioka H, Zhu X
Journal of cell science 2021 Jul 1;134(13)
Journal of cell science 2021 Jul 1;134(13)
The Novel ALG-2 Target Protein CDIP1 Promotes Cell Death by Interacting with ESCRT-I and VAPA/B.
Inukai R, Mori K, Kuwata K, Suzuki C, Maki M, Takahara T, Shibata H
International journal of molecular sciences 2021 Jan 25;22(3)
International journal of molecular sciences 2021 Jan 25;22(3)
VPS13D interacts with VCP/p97 and negatively regulates endoplasmic reticulum-mitochondria interactions.
Du Y, Wang J, Xiong J, Fang N, Ji WK
Molecular biology of the cell 2021 Aug 1;32(16):1474-1486
Molecular biology of the cell 2021 Aug 1;32(16):1474-1486
A High-Density Human Mitochondrial Proximity Interaction Network.
Antonicka H, Lin ZY, Janer A, Aaltonen MJ, Weraarpachai W, Gingras AC, Shoubridge EA
Cell metabolism 2020 Sep 1;32(3):479-497.e9
Cell metabolism 2020 Sep 1;32(3):479-497.e9
Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation.
Yamanaka T, Nishiyama R, Shimogori T, Nukina N
Scientific reports 2020 May 6;10(1):7610
Scientific reports 2020 May 6;10(1):7610
Nir2 Is an Effector of VAPs Necessary for Efficient Hepatitis C Virus Replication and Phosphatidylinositol 4-Phosphate Enrichment at the Viral Replication Organelle.
Wang H, Tai AW
Journal of virology 2019 Nov 15;93(22)
Journal of virology 2019 Nov 15;93(22)
A trap mutant reveals the physiological client spectrum of TRC40.
Coy-Vergara J, Rivera-Monroy J, Urlaub H, Lenz C, Schwappach B
Journal of cell science 2019 Jul 1;132(13)
Journal of cell science 2019 Jul 1;132(13)
MIGA2 Links Mitochondria, the ER, and Lipid Droplets and Promotes De Novo Lipogenesis in Adipocytes.
Freyre CAC, Rauher PC, Ejsing CS, Klemm RW
Molecular cell 2019 Dec 5;76(5):811-825.e14
Molecular cell 2019 Dec 5;76(5):811-825.e14
Long-chain acyl-CoA synthetase 1 interacts with key proteins that activate and direct fatty acids into niche hepatic pathways.
Young PA, Senkal CE, Suchanek AL, Grevengoed TJ, Lin DD, Zhao L, Crunk AE, Klett EL, Füllekrug J, Obeid LM, Coleman RA
The Journal of biological chemistry 2018 Oct 26;293(43):16724-16740
The Journal of biological chemistry 2018 Oct 26;293(43):16724-16740
Loss of VAPB Regulates Autophagy in a Beclin 1-Dependent Manner.
Wu D, Hao Z, Ren H, Wang G
Neuroscience bulletin 2018 Dec;34(6):1037-1046
Neuroscience bulletin 2018 Dec;34(6):1037-1046
DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.
Zheng P, Chen Q, Tian X, Qian N, Chai P, Liu B, Hu J, Blackstone C, Zhu D, Teng J, Chen J
Cell research 2018 Aug;28(8):833-854
Cell research 2018 Aug;28(8):833-854
Sequence-dependent cargo recognition by SNX-BARs mediates retromer-independent transport of CI-MPR.
Simonetti B, Danson CM, Heesom KJ, Cullen PJ
The Journal of cell biology 2017 Nov 6;216(11):3695-3712
The Journal of cell biology 2017 Nov 6;216(11):3695-3712
Adenovirus Modulates Toll-Like Receptor 4 Signaling by Reprogramming ORP1L-VAP Protein Contacts for Cholesterol Transport from Endosomes to the Endoplasmic Reticulum.
Cianciola NL, Chung S, Manor D, Carlin CR
Journal of virology 2017 Mar 15;91(6)
Journal of virology 2017 Mar 15;91(6)
The ATF6 pathway of the ER stress response contributes to enhanced viability in glioblastoma.
Dadey DY, Kapoor V, Khudanyan A, Urano F, Kim AH, Thotala D, Hallahan DE
Oncotarget 2016 Jan 12;7(2):2080-92
Oncotarget 2016 Jan 12;7(2):2080-92
The role of endoplasmic reticulum-related BiP/GRP78 in interferon gamma-induced persistent Chlamydia pneumoniae infection.
Shima K, Klinger M, Schütze S, Kaufhold I, Solbach W, Reiling N, Rupp J
Cellular microbiology 2015 Jul;17(7):923-34
Cellular microbiology 2015 Jul;17(7):923-34
Expression of vesicle-associated membrane-protein-associated protein B cleavage products in peripheral blood leukocytes and cerebrospinal fluid of patients with sporadic amyotrophic lateral sclerosis.
Deidda I, Galizzi G, Passantino R, Cascio C, Russo D, Colletti T, La Bella V, Guarneri P
European journal of neurology 2014 Mar;21(3):478-85
European journal of neurology 2014 Mar;21(3):478-85
Widespread aggregation of mutant VAPB associated with ALS does not cause motor neuron degeneration or modulate mutant SOD1 aggregation and toxicity in mice.
Qiu L, Qiao T, Beers M, Tan W, Wang H, Yang B, Xu Z
Molecular neurodegeneration 2013 Jan 3;8:1
Molecular neurodegeneration 2013 Jan 3;8:1
Disease mutations in Rab7 result in unregulated nucleotide exchange and inappropriate activation.
McCray BA, Skordalakes E, Taylor JP
Human molecular genetics 2010 Mar 15;19(6):1033-47
Human molecular genetics 2010 Mar 15;19(6):1033-47
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 14477-1-AP(VAPB antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using VAPB antibody 14477-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-VAPB (IP:14477-1-AP, 3ug; Detection:14477-1-AP 1:800) with HeLa cells lysate 1840ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human pancreas cancer using 14477-1-AP(VAPB antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human pancreas cancer using 14477-1-AP(VAPB antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The VAPB antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human VAPB. This antibody recognizes human,mouse,rat antigen. The VAPB antibody has been validated for the following applications: ELISA, WB, IHC, IF, IP, FC analysis.