14477-1-AP

antibody from Invitrogen Antibodies

Targeting: VAPB ALS8, VAP-B, VAP-C

Immunoprecipitation

Antibody data

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Validation data

Product number: 14477-1-AP - Provider product page
Provider: Invitrogen Antibodies
Product name: VAPB Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Other
Description: Immunogen sequence: KLGNPTDRN VCFKVKTTAP RRYCVRPNSG IIDAGASINV SVMLQPFDYD PNEKSKHKFM VQSMFAPTDT SDMEAVWKEA KPEDLMDSKL RCVFELPAEN DKPHDVEINK IISTTASKTE TPIVSKSLSS SLDDTEVKKV MEECKRLQGE V (31-180 aa encoded by BC001712)
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 150 µL
Concentration: 0.13 mg/mL
Storage: -20°C

VAPB protein structure - 14477-1-AP shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Mouse brain tissue were subjected to SDS PAGE followed by western blot with 14477-1-AP (VAPB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: WB result of VAPB antibody (14477-1-AP, 1:5000) with si-control and si-VAPB transfected HepG2 cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14477-1-AP (VAPB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HepG2 cells were subjected to SDS PAGE followed by western blot with 14477-1-AP (VAPB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Jurkat cells were subjected to SDS PAGE followed by western blot with 14477-1-AP (VAPB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Rat brain tissue were subjected to SDS PAGE followed by western blot with 14477-1-AP (VAPB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human pancreas cancer using 14477-1-AP (VAPB antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human pancreas cancer using 14477-1-AP (VAPB antibody) at dilution of 1:100 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human breast cancer slide using 14477-1-AP (VAPB Antibody) at dilution of 1:50.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human breast cancer slide using 14477-1-AP (VAPB Antibody) at dilution of 1:50.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 14477-1-AP (VAPB antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).