AF5855

antibody from R&D Systems

Targeting: VAPB ALS8, VAP-B, VAP-C

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF5855

Provider

R&D Systems

Product name

Human VAP-B Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human VAP-B in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant rat VAP-B is observed and less than 5% cross-reactivity with recombinant human VAP-A is observed.

Reactivity

Human

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

O95292


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human VAP-B by Western Blot. Western blot shows lysates of human skeletal muscle tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human VAP-B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5855) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for VAP-B at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

VAP-B in Human Brain. VAP-B was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Sheep Anti-Human VAP-B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5855) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.