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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- 200-301-E84 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#200-301-E84, RRID:AB_2611120
- Product name
- Anti-Alpha A Crystallin (MOUSE) Monoclonal Antibody - 200-301-E84
- Antibody type
- Monoclonal
- Vial size
- 200 µl
Submitted references A tamoxifen-inducible Cre knock-in mouse for lens-specific gene manipulation.
Wei Z, Hao C, Chen JK, Gan L, Fan X
Experimental eye research 2023 Jan;226:109306
Experimental eye research 2023 Jan;226:109306
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of mouse Anti-Alpha A Crystallin Antibody. Lane 1: AlphaA Crystallin Lane2: AlphaB Crystallin. Load: 20ug per lane. Primary antibody: AlphaA-Crystallin 1:1000 for overnight at 4°C . Secondary antibody: Goat anti-Mouse IgG HRP antibody at 1:40,000 for 45 min at RT. Block: 5% Blotto overnight at 4°C. Predicted/Observed size: 19.9 kDa, 20 kDa for Alpha A.
- Validation comment
- Western Blot
