PA5-118177

antibody from Invitrogen Antibodies

Targeting: PPAT GPAT, PRAT

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118177

Provider

Invitrogen Antibodies

Product name

PPAT Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PPAT Polyclonal Antibody (Product # PA5-118177) at 1:500 dilution. Lane A: HeLa Whole Cell Lysate, Lane B: A431 Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 57 kDa. (We are unsure of the identity of these extra bands).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PPAT Immunoprecipitation using: Lane A: 0.5 mg HeLa Whole Cell Lysate 4 µL with PPAT Polyclonal Antibody (Product # PA5-118177) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: PPAT Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1,000dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 62 kDa. Observed band size: 60 kDa.