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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-38399 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- eIF5A Recombinant Rabbit Monoclonal Antibody (5E1)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- This antibody has been tested in direct-ELISA
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 5E1
- Vial size
- 100 µL
- Concentration
- 0.09 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of eIF5A using eIF5A Monoclonal Antibody (Product # MA5-38399) at 1:2,000. Positive WB detected in: A2780 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, Raji whole cell lysate. Secondary: Goat polyclonal to rabbit IgG at 1:50,000 dilution. Predicted band size: 17, 21 kDa. Observed band size: 18 kDa
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence analysis of eIF5A in HeLa cells using eIF5A Monoclonal Antibody (Product # MA5-38399) diluted at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of eIF5A in paraffin-embedded human colon cancer using eIF5A Monoclonal Antibody (Product # MA5-38399) diluted at 1:100. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of eIF5A in Jurkat cells using eIF5A Monoclonal Antibody (Product # MA5-38399) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1 µg/1x10^6 cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1:200 dilution for 30min at 4°C. Control antibody (green line) was Rabbit IgG (1 µg/1x10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
