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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- MA1-23277 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RbAp46/RbAp48 Monoclonal Antibody (15G12)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Recommended positive controls: K562, THP-1, HL-60.
- Antibody clone number
- 15G12
- Concentration
- 1 mg/mL
Submitted references Alu elements in ANRIL non-coding RNA at chromosome 9p21 modulate atherogenic cell functions through trans-regulation of gene networks.
Dual retinoblastoma-binding proteins with properties related to a negative regulator of ras in yeast.
Holdt LM, Hoffmann S, Sass K, Langenberger D, Scholz M, Krohn K, Finstermeier K, Stahringer A, Wilfert W, Beutner F, Gielen S, Schuler G, Gäbel G, Bergert H, Bechmann I, Stadler PF, Thiery J, Teupser D
PLoS genetics 2013;9(7):e1003588
PLoS genetics 2013;9(7):e1003588
Dual retinoblastoma-binding proteins with properties related to a negative regulator of ras in yeast.
Qian YW, Lee EY
The Journal of biological chemistry 1995 Oct 27;270(43):25507-13
The Journal of biological chemistry 1995 Oct 27;270(43):25507-13
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RbAp46, 48 using 30 µg of A) Raji (B) K562 (C) THP-1 and D) HL-60 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a RbAp46, 48 monoclonal antibody (Product # MA1-23277) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RbAp46/RbAp48 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a RbAp46/RbAp48 Monoclonal Antibody (15G12) (Product # MA1-23277) at a dilution of 1:500 and a HRP-conjugated anti-mouse IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RbAp46/RbAp48 Monoclonal Antibody (15G12) (Product # MA1-23277). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with RbAp46/RbAp48 Monoclonal Antibody (15G12) (Product # MA1-23277) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of RbAp46/RbAp48 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: RbAp46/RbAp48 Monoclonal Antibody (15G12) (Product # MA1-23277) diluted at 1:500. Red: phalloidin, a cytoskeleton marker.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of RbAp46/RbAp48 was performed in paraffin-embedded human cervical carcinoma tissue using RbAp46/RbAp48 Monoclonal Antibody (15G12) (Product # MA1-23277) at a dilution of 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using RbAp48 + RbAp46 (Product # MA1-23277) antibody at 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using RbAp48 + RbAp46 (Product # MA1-23277) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 ANRIL binds to PRC1 and 2 proteins and recruits CBX7 and SUZ12 to promoters of target genes. (A, B) RNA immunoprecipitation (RIP) followed by qRT-PCR demonstrating ANRIL binding to PRC but not to CoREST/REST proteins in (A) ANRIL2 and (B) ANRIL4 cells. Copies of ANRIL relative to input control are given in (A) blue and (B) red, nuclear ncRNA U1 (white) was used as negative control. rIgG/mIgG/gIgG- rabbit/mouse/goat IgG controls. Error bars indicate s.e.m. (C,D) SUZ12 binding in promoters of ANRIL up-(green), down-(red), and not (black) regulated genes in (C) vector control cell line and (D) in BGO3 cells (GSM602674). TSS- transcription start site. (E, F) Effect of ANRIL over-expression on (E) SUZ12 and (F) CBX7 binding in promoters of up-regulated genes (vector control- dotted line vs. ANRIL2- straight line). (G) Reversal of ANRIL trans -regulation by RNAi against SUZ12 and CBX7 in ANRIL2 cells. SCR- scrambled siRNA control.
