NBP1-04261

antibody from Novus Biologicals

Targeting: AK3 AK3L1, AK6, AKL3L1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-04261

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-04261, RRID:AB_1520204

Product name

Mouse Monoclonal AK3 Antibody

Antibody type

Monoclonal

Description

Protein G purified.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: AK3 Antibody (SJB3-36) [NBP1-04261] - The recombinant human AK isozymes (AK1, AK2, AK3) and mouse liver were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-AK3 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: AK3 Antibody (SJB3-36) [NBP1-04261] - The recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AK3 antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Recombinant Human AK1 Lane 2. : Recombinant Human AK2 Lane 3. : Recombinant Human AK3

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: AK3 Antibody (SJB3-36) [NBP1-04261] - The lysate (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human AK3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Hep3B cell lysate Lane 2. : 293T cell lysate Lane 3. : Mouse kidney tissue lysate

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: AK3 Antibody (SJB3-36) [NBP1-04261] - The lysate (40ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human AK3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : Mouse liver tissue lysate

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: AK3 Antibody (SJB3-36) [NBP1-04261] - Human liver tissue was incubated with anti-human AK3 (1:100) for 2 hours at room temperature. Slide was then washed in PBS, and was incubated in avidin biosystem anti-rabbit labeled polymer for 30 min at RT. Enzyme detection was performed with DAB chromo-gen.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: AK3 Antibody (SJB3-36) [NBP1-04261] - Analysis of AK3 in jurkat cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).