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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00051806-M16 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00051806-M16, RRID:AB_1136904
- Product name
- CALML5 monoclonal antibody (M16), clone 2F10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant CALML5.
- Antigen sequence
MAGELTPEEEAQYKKAFSAVDTDGNGTINAQELGA
ALKATGKNLSEAQLRKLISEVDGDGDGEISFQEFL
TAARKARAGLEDLQVAFRAFDQDGDGHITVDELRR
AMAGLGQPLPQEELDAMIREADVDQDGRVNYEEFA
RMLAQE- Isotype
- IgG
- Antibody clone number
- 2F10
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of CALML5 expression in transfected 293T cell line by CALML5 monoclonal antibody (M16), clone 2F10.Lane 1: CALML5 transfected lysate(15.9 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CALML5 is 0.03 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to CALML5 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
