AP7262a

antibody from Abgent

Targeting: EIF4A1 DDX2A, EIF-4A, EIF4A

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP7262a

Provider

Abgent

Proper citation

Abgent Cat#AP7262a, RRID:AB_1208652

Product name

EIF4A1 Antibody (N-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

2 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of anti-EIF4A1 Pab (Cat. #AP7262a) in K562 cell line lysates (35ug/lane). EIF4A1(arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of EIF4A1 (arrow) using rabbit polyclonal EIF4A1 Antibody (N-term) (Cat.#AP7262a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4A1 gene (Lane 2) (Origene Technologies).

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EIF4A1 antibody (N-term) (Cat.#AP7262a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:10~50