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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00004752-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004752-M01, RRID:AB_540781
- Product name
- Mouse Monoclonal NEK3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. NEK3 - NIMA (never in mitosis gene a)-related kinase 3
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NEK3 Antibody (2F8) [H00004752-M01] - Analysis of NEK3 expression in transfected 293T cell line by NEK3 monoclonal antibody (M01), clone 2F8.Lane 1: NEK3 transfected lysate(57.7 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: NEK3 Antibody (2F8) [H00004752-M01] - Detection limit for recombinant GST tagged NEK3 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: NEK3 Antibody (2F8) [H00004752-M01] - Analysis of monoclonal antibody to NEK3 on HeLa cell. Antibody concentration 10 ug/ml.
