66011-1-IG

antibody from Invitrogen Antibodies

Targeting: EXOC2 FLJ11026, SEC5L1, Sec5p

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

66011-1-IG

Provider

Invitrogen Antibodies

Product name

SEC5/EXOC2 Monoclonal Antibody (1C11G2)

Antibody type

Monoclonal

Antigen

Other

Description

Immunogen sequence: TIQDLILDL RVRCVMATLQ HTAEEIKRLA EKEDWIVDNE GLTSLPCQFE QCIVCSLQSL KGVLECKPGE ASVFQQPKTQ EEVCQLSINI MQVFIYCLEQ LSTKPDADID TTHLSVDVSS PDLFGSIHED FSLTSEQRLL IVLSNCCYLE RHTFLNIAEH FEKHNFQGIE KITQVSMASL KELDQRLFEN YIELKADPIV GSLEPGIYAG YFDWKDCLPP TGVRNYLKEA LVNIIAVHAE VFTISKELVP RVLSKVIEAV SEELSRLMQC VSSFSKNGAL QARLEICALR DTVAVYLTPE SKSSFKQALE ALPQLSSGAD KKLLEELLNK FKSSMHLQLT CFQAASSTMM KT (456-806 aa encoded by BC016918)

Reactivity

Human, Mouse, Rat, Porcine

Host

Mouse

Isotype

IgG

Antibody clone number

1C11G2

Vial size

150 µL

Concentration

1.22 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 66011-1-IG (SEC5/EXOC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human brain tissue were subjected to SDS PAGE followed by western blot with 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

pig brain tissue were subjected to SDS PAGE followed by western blot with 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

rat brain tissue were subjected to SDS PAGE followed by western blot with 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SEC5/EXOC2 antibody (Product # 66011-1-IG, Clone: 1C11G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using 66011-1-IG (SEC5/EXOC2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66011-1-IG ( SEC5/EXOC2 antibody) at dilution of 1:400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66011-1-IG ( SEC5/EXOC2 antibody) at dilution of 1:400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 66011-1-Ig (SEC5/EXOC2 antibody) at dilution of 1:400 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-SEC5/EXOC2 (IP:66011-1-IG, 5ug; Detection:66011-1-IG 1:500) with mouse brain tissue lysate 3440ug.