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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [3]
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- Product number
- NBP1-37774 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-37774, RRID:AB_2069037
- Product name
- Goat Polyclonal Calnexin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Reported variants represent identical protein (NP_001019820.1, NP_001737.1).
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Bestrophin-3 is differently expressed in normal and injured mouse glomerular podocytes.
Assembly and misassembly of cystic fibrosis transmembrane conductance regulator: folding defects caused by deletion of F508 occur before and after the calnexin-dependent association of membrane spanning domain (MSD) 1 and MSD2.
Golubinskaya V, Elvin J, Ebefors K, Gustafsson H, Mallard C, Nyström J, Nilsson H
Acta physiologica (Oxford, England) 2015 Aug;214(4):481-96
Acta physiologica (Oxford, England) 2015 Aug;214(4):481-96
Assembly and misassembly of cystic fibrosis transmembrane conductance regulator: folding defects caused by deletion of F508 occur before and after the calnexin-dependent association of membrane spanning domain (MSD) 1 and MSD2.
Rosser MF, Grove DE, Chen L, Cyr DM
Molecular biology of the cell 2008 Nov;19(11):4570-9
Molecular biology of the cell 2008 Nov;19(11):4570-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Calnexin Antibody [NBP1-37774] - Staining of Mouse Heart lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Calnexin Antibody [NBP1-37774] - NBP1-37774 (1.5 ug/mL) as the reporter with another Calnexin antibody as the capture rabbit antibody (2.5 ug/mL).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-37774] - Staining of paraffin embedded Human Testis. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-37774] - Staining of paraffin embedded Human Kidney. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Calnexin Antibody [NBP1-37774] - Staining of paraffin embedded Human Uterus. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
