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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 600-401-B27 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-B27, RRID:AB_2209305
- Product name
- Anti-TRAF2 (RABBIT) Antibody - 600-401-B27
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's affinity purified anti-TRAF2 antibody shows detection of endogenous TRAF2 in whole HeLa cell lysates. Lane 2 shows endogenous TRAF2 detected with antibody at 47 kDa (arrowhead). Lane 4 shows no reactivity when blot is incubated with immunizing peptide. The identity of lower molecular weight band in lane 2 is unknown. Briefly, each lane contains approximately 14 µg of lysate. Membranes were blocked in 3% BSA-TBS 30 min. at room temperature. Primary antibody was used at a 1:500 dilution in 3% BSA-TBS and reacted overnight at 4°C. The membrane was washed and reacted with a 1:20,000 dilution conjugated Gt-a-Rabbit DyLight 649 (611-143-122) for 1 hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers in lanes 1 and 3. Fluorescence image was captured using the VersaDoc® Imaging System developed by Bio-Rad.
- Validation comment
- Western Blot
