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- Antibody Data
- Antigen structure
- References [40]
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- Product number
- NB110-93479 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-93479, RRID:AB_1237282
- Product name
- Rabbit Polyclonal Pyruvate Dehydrogenase E1-alpha subunit Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This is specific for the phosphorylated Serine 293 form of the PDHE1 alpha protein. It is recommended that BSA rather than non-fat milk be used in membrane blocking and antibody dilution.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Pyruvate dehydrogenase kinase is a negative regulator of interleukin-10 production in macrophages.
The novel function of tumor protein D54 in regulating pyruvate dehydrogenase and metformin cytotoxicity in breast cancer.
Akt1-mediated CPR cooling protection targets regulators of metabolism, inflammation and contractile function in mouse cardiac arrest.
Rational combination with PDK1 inhibition overcomes cetuximab resistance in head and neck squamous cell carcinoma.
The lipid droplet-associated protein ABHD5 protects the heart through proteolysis of HDAC4.
Alcohol inhibits T-cell glucose metabolism and hepatitis in ALDH2-deficient mice and humans: roles of acetaldehyde and glucocorticoids.
ORP4L Extracts and Presents PIP2 from Plasma Membrane for PLCβ3 Catalysis: Targeting It Eradicates Leukemia Stem Cells.
Mitochondrial Uncoupling Coordinated With PDH Activation Safely Ameliorates Hyperglycemia via Promoting Glucose Oxidation.
Loss of GCN5L1 in cardiac cells limits mitochondrial respiratory capacity under hyperglycemic conditions.
MiR-494-3p regulates mitochondrial biogenesis and thermogenesis through PGC1-α signalling in beige adipocytes.
The protein acetylase GCN5L1 modulates hepatic fatty acid oxidation activity via acetylation of the mitochondrial β-oxidation enzyme HADHA.
The mitochondrial calcium uniporter underlies metabolic fuel preference in skeletal muscle.
Early TCR Signaling Induces Rapid Aerobic Glycolysis Enabling Distinct Acute T Cell Effector Functions.
MicroRNA-23a promotes colorectal cancer cell survival by targeting PDK4.
Butyrate induces apoptosis by activating PDC and inhibiting complex I through SIRT3 inactivation.
PPARγ activation regulates lipid droplet formation and lactate production in rat Sertoli cells.
Adipose HIF-1α causes obesity by suppressing brown adipose tissue thermogenesis.
Pathological consequences of MICU1 mutations on mitochondrial calcium signalling and bioenergetics.
Down-regulation of PDK4 is Critical for the Switch of Carbohydrate Catabolism during Syncytialization of Human Placental Trophoblasts.
ORP4L is essential for T-cell acute lymphoblastic leukemia cell survival.
Reactive oxygen species (ROS) production triggered by prostaglandin D2 (PGD2) regulates lactate dehydrogenase (LDH) expression/activity in TM4 Sertoli cells.
ASCT2 (SLC1A5) is an EGFR-associated protein that can be co-targeted by cetuximab to sensitize cancer cells to ROS-induced apoptosis.
Key glycolytic enzyme activities of skeletal muscle are decreased under fed and fasted states in mice with knocked down levels of Shc proteins.
Novel molecular mechanisms involved in hormonal regulation of lactate production in Sertoli cells.
Dichloroacetate affects proliferation but not survival of human colorectal cancer cells.
Pyruvate dehydrogenase kinase expression and metabolic changes following dichloroacetate exposure in anoxic human colorectal cancer cells.
Warburg-like Glycolysis and Lactate Shuttle in Mouse Decidua during Early Pregnancy.
The regulation of neuronal mitochondrial metabolism by calcium.
Silencing gastrin-releasing peptide receptor suppresses key regulators of aerobic glycolysis in neuroblastoma cells.
Glycolytic switch in response to betulinic acid in non-cancer cells.
Activation of PPAR α and PPAR β/δ regulates Sertoli cell metabolism.
Shc depletion stimulates brown fat activity in vivo and in vitro.
Nutrient deprivation induces the Warburg effect through ROS/AMPK-dependent activation of pyruvate dehydrogenase kinase.
p53 negatively regulates transcription of the pyruvate dehydrogenase kinase Pdk2.
Two protein kinase C isoforms, δ and ε, regulate energy homeostasis in mitochondria by transmitting opposing signals to the pyruvate dehydrogenase complex.
Erk regulation of pyruvate dehydrogenase flux through PDK4 modulates cell proliferation.
The increase in cardiac pyruvate dehydrogenase kinase-4 after short-term dexamethasone is controlled by an Akt-p38-forkhead box other factor-1 signaling axis.
Control of oxidative phosphorylation by vitamin A illuminates a fundamental role in mitochondrial energy homoeostasis.
Regulation of intermediary metabolism by the PKCdelta signalosome in mitochondria.
Pyruvate dehydrogenase complex activity controls metabolic and malignant phenotype in cancer cells.
Na YR, Jung D, Song J, Park JW, Hong JJ, Seok SH
Journal of molecular cell biology 2020 Jul 1;12(7):543-555
Journal of molecular cell biology 2020 Jul 1;12(7):543-555
The novel function of tumor protein D54 in regulating pyruvate dehydrogenase and metformin cytotoxicity in breast cancer.
Zhuang Y, Ly RC, Frazier CV, Yu J, Qin S, Fan XY, Goetz MP, Boughey JC, Weinshilboum R, Wang L
Cancer & metabolism 2019;7:1
Cancer & metabolism 2019;7:1
Akt1-mediated CPR cooling protection targets regulators of metabolism, inflammation and contractile function in mouse cardiac arrest.
Li J, Zhu X, Wang H, Lee C, Chen SJ, Qian Y, Han M, Bunney R, Beiser DG, Vanden Hoek TL
PloS one 2019;14(8):e0220604
PloS one 2019;14(8):e0220604
Rational combination with PDK1 inhibition overcomes cetuximab resistance in head and neck squamous cell carcinoma.
Lu H, Lu Y, Xie Y, Qiu S, Li X, Fan Z
JCI insight 2019 Oct 3;4(19)
JCI insight 2019 Oct 3;4(19)
The lipid droplet-associated protein ABHD5 protects the heart through proteolysis of HDAC4.
Jebessa ZH, Shanmukha KD, Dewenter M, Lehmann LH, Xu C, Schreiter F, Siede D, Gong XM, Worst BC, Federico G, Sauer SW, Fischer T, Wechselberger L, Müller OJ, Sossalla S, Dieterich C, Most P, Gröne HJ, Moro C, Oberer M, Haemmerle G, Katus HA, Tyedmers J, Backs J
Nature metabolism 2019 Nov;1(11):1157-1167
Nature metabolism 2019 Nov;1(11):1157-1167
Alcohol inhibits T-cell glucose metabolism and hepatitis in ALDH2-deficient mice and humans: roles of acetaldehyde and glucocorticoids.
Gao Y, Zhou Z, Ren T, Kim SJ, He Y, Seo W, Guillot A, Ding Y, Wu R, Shao S, Wang X, Zhang H, Wang W, Feng D, Xu M, Han E, Zhong W, Zhou Z, Pacher P, Niu J, Gao B
Gut 2019 Jul;68(7):1311-1322
Gut 2019 Jul;68(7):1311-1322
ORP4L Extracts and Presents PIP2 from Plasma Membrane for PLCβ3 Catalysis: Targeting It Eradicates Leukemia Stem Cells.
Zhong W, Xu M, Li C, Zhu B, Cao X, Li D, Chen H, Hu C, Li R, Luo C, Pan G, Zhang W, Lai C, Wang T, Du X, Chen H, Xu G, Olkkonen VM, Lei P, Xu J, Yan D
Cell reports 2019 Feb 19;26(8):2166-2177.e9
Cell reports 2019 Feb 19;26(8):2166-2177.e9
Mitochondrial Uncoupling Coordinated With PDH Activation Safely Ameliorates Hyperglycemia via Promoting Glucose Oxidation.
Jiang H, Jin J, Duan Y, Xie Z, Li Y, Gao A, Gu M, Zhang X, Peng C, Xia C, Dong T, Li H, Yu L, Tang J, Yang F, Li J, Li J
Diabetes 2019 Dec;68(12):2197-2209
Diabetes 2019 Dec;68(12):2197-2209
Loss of GCN5L1 in cardiac cells limits mitochondrial respiratory capacity under hyperglycemic conditions.
Thapa D, Zhang M, Manning JR, Guimarães DA, Stoner MW, Lai YC, Shiva S, Scott I
Physiological reports 2019 Apr;7(8):e14054
Physiological reports 2019 Apr;7(8):e14054
MiR-494-3p regulates mitochondrial biogenesis and thermogenesis through PGC1-α signalling in beige adipocytes.
Lemecha M, Morino K, Imamura T, Iwasaki H, Ohashi N, Ida S, Sato D, Sekine O, Ugi S, Maegawa H
Scientific reports 2018 Oct 10;8(1):15096
Scientific reports 2018 Oct 10;8(1):15096
The protein acetylase GCN5L1 modulates hepatic fatty acid oxidation activity via acetylation of the mitochondrial β-oxidation enzyme HADHA.
Thapa D, Wu K, Stoner MW, Xie B, Zhang M, Manning JR, Lu Z, Li JH, Chen Y, Gucek M, Playford MP, Mehta NN, Harmon D, O'Doherty RM, Jurczak MJ, Sack MN, Scott I
The Journal of biological chemistry 2018 Nov 16;293(46):17676-17684
The Journal of biological chemistry 2018 Nov 16;293(46):17676-17684
The mitochondrial calcium uniporter underlies metabolic fuel preference in skeletal muscle.
Kwong JQ, Huo J, Bround MJ, Boyer JG, Schwanekamp JA, Ghazal N, Maxwell JT, Jang YC, Khuchua Z, Shi K, Bers DM, Davis J, Molkentin JD
JCI insight 2018 Nov 15;3(22)
JCI insight 2018 Nov 15;3(22)
Early TCR Signaling Induces Rapid Aerobic Glycolysis Enabling Distinct Acute T Cell Effector Functions.
Menk AV, Scharping NE, Moreci RS, Zeng X, Guy C, Salvatore S, Bae H, Xie J, Young HA, Wendell SG, Delgoffe GM
Cell reports 2018 Feb 6;22(6):1509-1521
Cell reports 2018 Feb 6;22(6):1509-1521
MicroRNA-23a promotes colorectal cancer cell survival by targeting PDK4.
Deng YH, Deng ZH, Hao H, Wu XL, Gao H, Tang SH, Tang H
Experimental cell research 2018 Dec 15;373(1-2):171-179
Experimental cell research 2018 Dec 15;373(1-2):171-179
Butyrate induces apoptosis by activating PDC and inhibiting complex I through SIRT3 inactivation.
Xu S, Liu CX, Xu W, Huang L, Zhao JY, Zhao SM
Signal transduction and targeted therapy 2017;2:16035
Signal transduction and targeted therapy 2017;2:16035
PPARγ activation regulates lipid droplet formation and lactate production in rat Sertoli cells.
Gorga A, Rindone GM, Regueira M, Pellizzari EH, Camberos MC, Cigorraga SB, Riera MF, Galardo MN, Meroni SB
Cell and tissue research 2017 Sep;369(3):611-624
Cell and tissue research 2017 Sep;369(3):611-624
Adipose HIF-1α causes obesity by suppressing brown adipose tissue thermogenesis.
Jun JC, Devera R, Unnikrishnan D, Shin MK, Bevans-Fonti S, Yao Q, Rathore A, Younas H, Halberg N, Scherer PE, Polotsky VY
Journal of molecular medicine (Berlin, Germany) 2017 Mar;95(3):287-297
Journal of molecular medicine (Berlin, Germany) 2017 Mar;95(3):287-297
Pathological consequences of MICU1 mutations on mitochondrial calcium signalling and bioenergetics.
Bhosale G, Sharpe JA, Koh A, Kouli A, Szabadkai G, Duchen MR
Biochimica et biophysica acta. Molecular cell research 2017 Jun;1864(6):1009-1017
Biochimica et biophysica acta. Molecular cell research 2017 Jun;1864(6):1009-1017
Down-regulation of PDK4 is Critical for the Switch of Carbohydrate Catabolism during Syncytialization of Human Placental Trophoblasts.
Liu X, Zuo R, Bao Y, Qu X, Sun K, Ying H
Scientific reports 2017 Aug 16;7(1):8474
Scientific reports 2017 Aug 16;7(1):8474
ORP4L is essential for T-cell acute lymphoblastic leukemia cell survival.
Zhong W, Yi Q, Xu B, Li S, Wang T, Liu F, Zhu B, Hoffmann PR, Ji G, Lei P, Li G, Li J, Li J, Olkkonen VM, Yan D
Nature communications 2016 Sep 1;7:12702
Nature communications 2016 Sep 1;7:12702
Reactive oxygen species (ROS) production triggered by prostaglandin D2 (PGD2) regulates lactate dehydrogenase (LDH) expression/activity in TM4 Sertoli cells.
Rossi SP, Windschüttl S, Matzkin ME, Rey-Ares V, Terradas C, Ponzio R, Puigdomenech E, Levalle O, Calandra RS, Mayerhofer A, Frungieri MB
Molecular and cellular endocrinology 2016 Oct 15;434:154-65
Molecular and cellular endocrinology 2016 Oct 15;434:154-65
ASCT2 (SLC1A5) is an EGFR-associated protein that can be co-targeted by cetuximab to sensitize cancer cells to ROS-induced apoptosis.
Lu H, Li X, Lu Y, Qiu S, Fan Z
Cancer letters 2016 Oct 10;381(1):23-30
Cancer letters 2016 Oct 10;381(1):23-30
Key glycolytic enzyme activities of skeletal muscle are decreased under fed and fasted states in mice with knocked down levels of Shc proteins.
Hagopian K, Tomilov AA, Kim K, Cortopassi GA, Ramsey JJ
PloS one 2015;10(4):e0124204
PloS one 2015;10(4):e0124204
Novel molecular mechanisms involved in hormonal regulation of lactate production in Sertoli cells.
Regueira M, Artagaveytia SL, Galardo MN, Pellizzari EH, Cigorraga SB, Meroni SB, Riera MF
Reproduction (Cambridge, England) 2015 Oct;150(4):311-21
Reproduction (Cambridge, England) 2015 Oct;150(4):311-21
Dichloroacetate affects proliferation but not survival of human colorectal cancer cells.
Delaney LM, Ho N, Morrison J, Farias NR, Mosser DD, Coomber BL
Apoptosis : an international journal on programmed cell death 2015 Jan;20(1):63-74
Apoptosis : an international journal on programmed cell death 2015 Jan;20(1):63-74
Pyruvate dehydrogenase kinase expression and metabolic changes following dichloroacetate exposure in anoxic human colorectal cancer cells.
Ho N, Coomber BL
Experimental cell research 2015 Feb 1;331(1):73-81
Experimental cell research 2015 Feb 1;331(1):73-81
Warburg-like Glycolysis and Lactate Shuttle in Mouse Decidua during Early Pregnancy.
Zuo RJ, Gu XW, Qi QR, Wang TS, Zhao XY, Liu JL, Yang ZM
The Journal of biological chemistry 2015 Aug 28;290(35):21280-91
The Journal of biological chemistry 2015 Aug 28;290(35):21280-91
The regulation of neuronal mitochondrial metabolism by calcium.
Llorente-Folch I, Rueda CB, Pardo B, Szabadkai G, Duchen MR, Satrustegui J
The Journal of physiology 2015 Aug 15;593(16):3447-62
The Journal of physiology 2015 Aug 15;593(16):3447-62
Silencing gastrin-releasing peptide receptor suppresses key regulators of aerobic glycolysis in neuroblastoma cells.
Rellinger EJ, Romain C, Choi S, Qiao J, Chung DH
Pediatric blood & cancer 2015 Apr;62(4):581-6
Pediatric blood & cancer 2015 Apr;62(4):581-6
Glycolytic switch in response to betulinic acid in non-cancer cells.
Heiss EH, Kramer MP, Atanasov AG, Beres H, Schachner D, Dirsch VM
PloS one 2014;9(12):e115683
PloS one 2014;9(12):e115683
Activation of PPAR α and PPAR β/δ regulates Sertoli cell metabolism.
Regueira M, Riera MF, Galardo MN, Pellizzari EH, Cigorraga SB, Meroni SB
Molecular and cellular endocrinology 2014 Jan 25;382(1):271-281
Molecular and cellular endocrinology 2014 Jan 25;382(1):271-281
Shc depletion stimulates brown fat activity in vivo and in vitro.
Tomilov A, Bettaieb A, Kim K, Sahdeo S, Tomilova N, Lam A, Hagopian K, Connell M, Fong J, Rowland D, Griffey S, Ramsey J, Haj F, Cortopassi G
Aging cell 2014 Dec;13(6):1049-58
Aging cell 2014 Dec;13(6):1049-58
Nutrient deprivation induces the Warburg effect through ROS/AMPK-dependent activation of pyruvate dehydrogenase kinase.
Wu CA, Chao Y, Shiah SG, Lin WW
Biochimica et biophysica acta 2013 May;1833(5):1147-56
Biochimica et biophysica acta 2013 May;1833(5):1147-56
p53 negatively regulates transcription of the pyruvate dehydrogenase kinase Pdk2.
Contractor T, Harris CR
Cancer research 2012 Jan 15;72(2):560-7
Cancer research 2012 Jan 15;72(2):560-7
Two protein kinase C isoforms, δ and ε, regulate energy homeostasis in mitochondria by transmitting opposing signals to the pyruvate dehydrogenase complex.
Gong J, Hoyos B, Acin-Perez R, Vinogradov V, Shabrova E, Zhao F, Leitges M, Fischman D, Manfredi G, Hammerling U
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2012 Aug;26(8):3537-49
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2012 Aug;26(8):3537-49
Erk regulation of pyruvate dehydrogenase flux through PDK4 modulates cell proliferation.
Grassian AR, Metallo CM, Coloff JL, Stephanopoulos G, Brugge JS
Genes & development 2011 Aug 15;25(16):1716-33
Genes & development 2011 Aug 15;25(16):1716-33
The increase in cardiac pyruvate dehydrogenase kinase-4 after short-term dexamethasone is controlled by an Akt-p38-forkhead box other factor-1 signaling axis.
Puthanveetil P, Wang Y, Wang F, Kim MS, Abrahani A, Rodrigues B
Endocrinology 2010 May;151(5):2306-18
Endocrinology 2010 May;151(5):2306-18
Control of oxidative phosphorylation by vitamin A illuminates a fundamental role in mitochondrial energy homoeostasis.
Acin-Perez R, Hoyos B, Zhao F, Vinogradov V, Fischman DA, Harris RA, Leitges M, Wongsiriroj N, Blaner WS, Manfredi G, Hammerling U
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Feb;24(2):627-36
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Feb;24(2):627-36
Regulation of intermediary metabolism by the PKCdelta signalosome in mitochondria.
Acin-Perez R, Hoyos B, Gong J, Vinogradov V, Fischman DA, Leitges M, Borhan B, Starkov A, Manfredi G, Hammerling U
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Dec;24(12):5033-42
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Dec;24(12):5033-42
Pyruvate dehydrogenase complex activity controls metabolic and malignant phenotype in cancer cells.
McFate T, Mohyeldin A, Lu H, Thakar J, Henriques J, Halim ND, Wu H, Schell MJ, Tsang TM, Teahan O, Zhou S, Califano JA, Jeoung NH, Harris RA, Verma A
The Journal of biological chemistry 2008 Aug 15;283(33):22700-8
The Journal of biological chemistry 2008 Aug 15;283(33):22700-8
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [NB110-93479] - Detection of PDHE1 alpha [S293] in an in vitro autophosphorylation of PDH complex in response to different stimulants: (-) none, H202-hydrogen peroxide, AR-anhydroretinol, Rol-retinol, RA-retinoic acid, PMA, Lipid-PKC lipid activator. Photo courtesy of Dr. Beatrice Hoyos, Memorial Sloan-Kettering Cancer Center.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [NB110-93479] - Rate of aerobic glycolysis in the presence of BA.MEF were treated with 10 uM BA or DMSO (0.1%) for 16 h before they were subjected to a glycolysis stress test as described under "Materials and Methods". MEF were treated with DMSO (0.1%, D) and 10 uM BA for the indicated periods of time before total cell lysates were subjected to immunoblot analyses for pPDHE1 (Ser273) and total PDHE1 (molecular weight 43 kDa). Representative blots out of three experiments are shown. The graph below depicts compiled densitometric values of pPDHE/PDHE. Representative blots out of three independent experiments are shown. The graph below depicts compiled densitometric values of GLUT1/actin and GLUT3/actin, respectively. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0115683) licensed under a CC-BY licence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Pyruvate Dehydrogenase E1-alpha subunit [p Ser293] Antibody [NB110-93479] - PDH activity and protein levels of PDK4 and p-PDH in hindlimb skeletal muscle. Samples were resolved by electrophoresis, transferred and probed by antibodies and representative immunoblots and normalization of pPDH are presented. The following comparisons were made: within a genotype, fed versus fasted; across genotypes, fed versus fed and fasted versus fasted. Bars that do not share a common symbol differ significantly (P < 0.05). Data presented as mean +/- SEM (n = 6). Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0124204), licensed under a CC-BY licence.
