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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [2]
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- Product number
- RQ5984 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- LDLR Antibody / LDL Receptor
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Storage
- After reconstitution, the LDL Receptor antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HeLa, 2) human Raji, 3) human U-87 MG, 4) rat lung, 5) rat kidney and 6) rat RH35 lysate with LDL Receptor antibody. Expected molecular weight: 95-160 kDa depending on glycosylation level.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human rectal cancer with LDL Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human liver cancer with LDL Receptor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human A431 cells with LDL Receptor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LDL Receptor antibody.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human NRK cells with LDL Receptor antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= LDL Receptor antibody.
