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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00005100-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005100-M01, RRID:AB_546811
- Product name
- Mouse Monoclonal Protocadherin-8 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PCDH8 - protocadherin 8
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Identification of cell surface targets through meta-analysis of microarray data.
Identification of cell surface targets through meta-analysis of microarray data.
Haeberle H, Dudley JT, Liu JT, Butte AJ, Contag CH
Neoplasia (New York, N.Y.) 2012 Jul;14(7):666-9
Neoplasia (New York, N.Y.) 2012 Jul;14(7):666-9
Identification of cell surface targets through meta-analysis of microarray data.
Haeberle H, Dudley JT, Liu JT, Butte AJ, Contag CH
Neoplasia (New York, N.Y.) 2012 Jul;14(7):666-9
Neoplasia (New York, N.Y.) 2012 Jul;14(7):666-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Protocadherin-8 Antibody (6A8) [H00005100-M01] - Analysis of PCDH8 over-expressed 293 cell line, cotransfected with PCDH8 Validated Chimera RNAi ( Cat # H00005100-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PCDH8 monoclonal antibody (M01), clone 6A8 (Cat # H00005100-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Protocadherin-8 Antibody (6A8) [H00005100-M01] - Analysis of PCDH8 expression in transfected 293T cell line by PCDH8 monoclonal antibody (M01), clone 6A8.Lane 1: PCDH8 transfected lysate(113 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Protocadherin-8 Antibody (6A8) [H00005100-M01] - PCDH8 monoclonal antibody (M01), clone 6A8 Analysis of PCDH8 expression in COLO 320 HSR.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Protocadherin-8 Antibody (6A8) [H00005100-M01] - Detection limit for recombinant GST tagged PCDH8 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Protocadherin-8 Antibody (6A8) [H00005100-M01] - Analysis of monoclonal antibody to PCDH8 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
