Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-19613 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-19613, RRID:AB_1641869
- Product name
- Rabbit Polyclonal DEC2/SHARP1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references SHARP1 suppresses angiogenesis of endometrial cancer by decreasing hypoxia-inducible factor-1α level.
GLI1 interferes with the DNA mismatch repair system in pancreatic cancer through BHLHE41-mediated suppression of MLH1.
Liao Y, Lu W, Che Q, Yang T, Qiu H, Zhang H, He X, Wang J, Qiu M, Zou Y, Gu W, Wan X
PloS one 2014;9(6):e99907
PloS one 2014;9(6):e99907
GLI1 interferes with the DNA mismatch repair system in pancreatic cancer through BHLHE41-mediated suppression of MLH1.
Inaguma S, Riku M, Hashimoto M, Murakami H, Saga S, Ikeda H, Kasai K
Cancer research 2013 Dec 15;73(24):7313-23
Cancer research 2013 Dec 15;73(24):7313-23
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: DEC2/SHARP1 Antibody [NBP1-19613] - Image shows a specific band for SHARP1 in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DEC2/SHARP1 Antibody [NBP1-19613] - Analysis of HeLa (1), Raw 264.7 (2), A431 (3), MCF7 (4), and PC12 (5) using SHARP1 antibody at 2 ug/mL.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DEC2/SHARP1 Antibody [NBP1-19613] - Analysis of SHARP1 in HeLa whole cell extract.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: DEC2/SHARP1 Antibody [NBP1-19613] - Huh7 cell lysates loaded from left to right at concentrations of 20, 30, and 40 ug of total protein. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: DEC2/SHARP1 Antibody [NBP1-19613] - Staining of SHARP1 in mouse pancreas.