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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [2]
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- Product number
- PA5-22032 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CRYBA4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, H1299, Mouse brain.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Beta-crystallin A4 using 30 µg of A) H1299 and B) HeLa lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a Beta-crystallin A4 polyclonal antibody (Product # PA5-22032) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CRYBA4 was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a CRYBA4 Polyclonal Antibody (Product # PA5-22032) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CRYBA4 Polyclonal Antibody (Product # PA5-22032). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. CRYBA4 Polyclonal Antibody (Product # PA5-22032) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CRYBA4 was performed by separating 30 µg of whole cell extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a CRYBA4 Polyclonal Antibody (Product # PA5-22032) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Beta-crystallin A4 in paraformaldehyde-fixed HeLa cells using a Beta-crystallin A4 polyclonal antibody (Product # PA5-22032) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Beta-crystallin A4 antibody (Product # PA5-22032) at 1:200 dilution.
