Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunoprecipitation [1]
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Validation data
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- Product number
- ASM24 - Provider product page
- Provider
- Cytoskeleton, Inc.
- Product name
- Anti-SUMO-2/3
- Antibody type
- Monoclonal
- Antigen
- Full-length recombinant SUMO-2 protein (Uniprot: P61956) combined with a proprietary mix of peptides that include CQIRFRFDGQPINE.
- Description
- Anti-SUMO-2/3 antibody is a mouse monoclonal antibody. The antibody was raised against full-length recombinant SUMO-2 protein (Uniprot: P61956) combined with a proprietary mix of peptides that include CQIRFRFDGQPINE. The antibody has been shown to immunoprecipitate a wide range of SUMO-2/3 targeted proteins in a HeLa cell lysate (Fig. 1A). A linear epitope has not been identified and ASM24 appears to recognize a conformational epitope. Each Lot of antibody is quality controlled to provide a high batch to batch consistency. The Lot specific µg per tube can be found in the Lot specific COA documents at www.cytoskeleton.com. ASM24 is purified by Protein G affinity chromatography and is supplied as a lyophilized white powder. Each Lot of antibody is quality controlled to provide a high batch to batch consistency. The Lot specific µg per tube can be found in the Lot specific COA documents at www.cytoskeleton.com.
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Full-length recombinant SUMO-2 prot
ein (Uniprot: P61956) combined with
a proprietary mix of peptides that
include CQIRFRFDGQPINE.- Epitope
- unidentified
- Isotype
- IgG
- Antibody clone number
- 11G2
- Vial size
- 2 x 200µl
- Concentration
- Specified in COA
- Storage
- Lyophilized 4 °C. Reconstituted -20 °C.
- Handling
- Shipped at room temperture. The lyophilized protein can be stored desiccated at 4 °C for 6 months. Store at -20 °C when reconstituted.
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Supportive validation
- Submitted by
- Cytoskeleton, Inc. (provider)
- Main image
- Experimental details
- HeLa cells were stained and visualized by widefield fluorescence microscopy as de-scribed in the IF method below. The cells were stained against β-tubulin (sheep anti-tubulin Ab, Cat# ATN02, green) and SUMO-2/3 (11G2, red). DNA was stained with DAPI. Cells in interphase were imaged with a Zeiss Axio Observer.Z1 microscope (1.4 NA 63X objective). PML nuclear bodies (nuclear dots) were visible in SUMO-2/3 staining as has been previously reported5. To see the full Immunofluorescence protocol, see the product datasheet.
- Sample type
- HeLa cells
- Primary Ab dilution
- 1:500
- Secondary Ab
- fluorescently-labeled donkey anti-mouse
- Secondary Ab dilution
- 1:500
- Protocol
- Protocol
- Submitted by
- Cytoskeleton, Inc. (provider)
- Main image
- Experimental details
- HeLa cells were stained and visualized by widefield fluorescence microscopy as de-scribed in the IF method below. The cells were stained against α/β-tubulin (sheep anti-tubulin Ab, Cat# ATN02, green) and SUMO-2/3 (11G2, red). DNA was stained with DAPI. Mitotic cells in metaphase were imaged with a Zeiss Axio Observer.Z1 microscope (1.4 NA 63X objective). The localization of SUMO 2/3-conjugates at chromosomes can be observed during mitosis as has been previously reported12. To see the full Immunofluorescence protocol, see the product datasheet.
- Sample type
- HeLa cells
- Primary Ab dilution
- 1:500
- Secondary Ab
- fluorescently-labeled donkey anti-mouse
- Secondary Ab dilution
- 1:500
- Protocol
- Protocol
Supportive validation
- Submitted by
- Cytoskeleton, Inc. (provider)
- Main image
- Experimental details
- Denatured cell lysates were prepared from HS43, CT37 and KD S210 (HS43: Heat Shock treated (43°C for 10min), CT37: untreated and KD S2: shRNA SUMO-2 knock down). 1mg of lysate was used for the immunoprecipitation of SUMO-2/3 conjugates. IP experiments were performed by the protocol presented in IP and WB Method. Western blots of immunopre-cipitated proteins were developed using anti-SUMO-2/3 (Cytoskeleon cat# ASM23) (A) or anti-TFII-I antibody (B). (A) Star (*) and circle (o) indicate heavy and light chains of antibodies. Un-conjugated free SUMO is denoted by a triangle. (B) Unconjugated TFII-I is visible near 120kDa. Multiple bands indicate that TFII-I is SUMOylated by several SUMO-2/3 proteins. TFII-I has previously been reported to be a target for Sumoylation 10,11. To see the full Immunoprecipitation protocol, see the product datasheet.
- Sample type
- Denatured cell lysates were prepared from HS43, CT37 and KD S2
- Validation comment
- Supportive
- Primary Ab dilution
- Assay Dependent
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:20000
- Protocol
- Protocol