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Western blotAntibody data
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- References [1]
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- Validations
- Western blot [3]
- Flow cytometry [1]
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- Product number
- 40-1900 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SUMO-3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.25 mg/mL
- Storage
- -20°C
Submitted references Histone deacetylase 7 promotes PML sumoylation and is essential for PML nuclear body formation.
Gao C, Ho CC, Reineke E, Lam M, Cheng X, Stanya KJ, Liu Y, Chakraborty S, Shih HM, Kao HY
Molecular and cellular biology 2008 Sep;28(18):5658-67
Molecular and cellular biology 2008 Sep;28(18):5658-67
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SK-OV-3 cell lysates using Zymed Rb anti-SUMO-3 (Product # 40-1900).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-SUMO-3 Polyclonal Antibody (Product # 40-1900) and a 20 kDa band corresponding to free SUMO-3 was observed in PC-3 and Mouse Testis. The sumoylated form from 40-160 kDa has been observed in across cell lines and tissue extract tested. Whole cell extracts (30 µg lysate) of DU 145 (Lane 1), PC-3 (Lane 2) and tissue extract of Mouse Testis (Lane 3) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SK-OV-3 cell lysates using Zymed Rb anti-SUMO-3 (Product # 40-1900).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of SUMO-3 was done on Jurkat cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with SUMO-3 Rabbit Polyclonal Antibody (401900, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
