200-401-491

antibody from Rockland Immunochemicals, Inc.

Targeting: SUMO3 SMT3A, SMT3H1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

200-401-491

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#200-401-491, RRID:AB_828424

Product name

Anti-SUMO-3 (RABBIT) Antibody - 200-401-491

Antibody type

Polyclonal

Vial size

500 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot analysis is shown using Rockland's Affinity Purified anti-Human SUMO-3 antibody to detect GFP-SUMO fusion proteins (arrowheads). Panel A. Increasing concentrations of human GFP-SUMO-3 were run on a SDS-PAGE, transferred onto nitrocellulose, and blocked for 1 hour with 5% non-fat dry milk in TTBS, and probed overnight at 4°C with a 1:1000 dilution of anti-hSUMO-3 antibody in 5% non-fat dry milk in TTBS. Detection occurred using a 1:1,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). Panel B. Specificity of the antibody was confirmed by SDS-PAGE of 5 µg of various GFP-SUMO constructs followed by transfer onto nitrocellulose. Lanes: 1. MW marker, 2. GFP-human SUMO-3, 3. GFP-human SUMO-1, 4. GFP-yeast SUMO, 5. GFP-Arabidopsis thaliana, SUMO-1, 6. GFP- Arabidopsis thaliana SUMO-2, 7. GFP-tomato SUMO. After blocking for 1 hour with 5% non-fat dry milk in TTBS, the blot was probed overnight at 4°C with anti-hSUMO-3 antibody diluted and detected as above. Only the human GFP-SUMO-3 band was visualized by chemiluminescence, and no crossreactivity with other SUMO family members was observed.

Validation comment

Western Blot