Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-14268 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MOB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with mouse and rat based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Association of Mps one binder kinase activator 1 (MOB1) expression with poor disease-free survival in individuals with non-small cell lung cancer.
Ando N, Tanaka K, Otsubo K, Toyokawa G, Ikematsu Y, Ide M, Yoneshima Y, Iwama E, Inoue H, Ijichi K, Tagawa T, Nakanishi Y, Okamoto I
Thoracic cancer 2020 Oct;11(10):2830-2839
Thoracic cancer 2020 Oct;11(10):2830-2839
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MOBKL1B (arrow) using a MOBKL1B polyclonal antibody (Product # PA5-14268) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MOBKL1B gene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MOBKL1B (arrow) using a MOBKL1B polyclonal antibody (Product # PA5-14268) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MOBKL1B gene.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a MOBKL1B polyclonal antibody (Product # PA5-14268) in A2058 cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human prostate carcinoma using a MOBKL1B polyclonal antibody (Product # PA5-14268), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of A2058 cells using a MOBKL1B polyclonal antibody (Product # PA5-14268) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Representative immunohistochemical staining of MOB1 in lung adenocarcinoma specimens. Specimens negative (score = 0) ( a and b ) or positive (score = 4) ( c and d ) for MOB1 staining are shown at low ( a and c ) and high ( b and d ) magnification. Scale bars: 100 mum ( a and c ) or 50 mum ( b and d ).