11703-1-AP

antibody from Invitrogen Antibodies

Targeting: MCM5 CDC46

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11703-1-AP

Provider

Invitrogen Antibodies

Product name

MCM5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: SQLLKFVEK CSPIGVYTSG KGSSAAGLTA SVMRDPSSRN FIMEGGAMVL ADGGVVCIDE FDKMREDDRV AIHEAMEQQT ISIAKAGITT TLNSRCSVLA AANSVFGRWD ETKGEDNIDF MPTILSRFDM IFIVKDEHNE ERDVMLAKHV ITLHVSALTQ TQAVEGEIDL AKLKKFIAYC RVKCGPRLSA EAAEKLKNRY IIMRSGARQH ERDSDRRSSI PITVRQLEAI VRIAEALSKM KLQPFATEAD VEEALRLFQV STLDAALSGT LSGVEGFTSQ EDQEMLSRIE KQLKRRFAIG SQVSEHSIIK DFTKQKYPEH AIHKVLQLML RRGEIQHRMQ RKVLYRLK (388-735 aa encoded by BC000142)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 11703-1-AP (MCM5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11703-1-AP (MCM5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 11703-1-AP (MCM5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 11703-1-AP (MCM5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lymphoma using 11703-1-AP (MCM5 antibody) at dilution of 1:100 (under 10x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-MCM5 (IP:11703-1-AP, 4ug; Detection:11703-1-AP 1:500) with Jurkat cells lysate 2400ug.