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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NB100-79778 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-79778, RRID:AB_1109282
- Product name
- Rabbit Polyclonal MCM5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Basal level of FANCD2 monoubiquitination is required for the maintenance of a sufficient number of licensed-replication origins to fire at a normal rate.
Cyclin-dependent kinases regulate lysosomal degradation of hypoxia-inducible factor 1α to promote cell-cycle progression.
A nontranscriptional role for HIF-1α as a direct inhibitor of DNA replication.
MCM proteins are negative regulators of hypoxia-inducible factor 1.
TopBP1 and DNA polymerase-alpha directly recruit the 9-1-1 complex to stalled DNA replication forks.
Direct requirement for Xmus101 in ATR-mediated phosphorylation of Claspin bound Chk1 during checkpoint signaling.
Panneerselvam J, Pickering A, Han B, Li L, Zheng J, Zhang J, Zhang Y, Fei P
Oncotarget 2014 Mar 15;5(5):1326-37
Oncotarget 2014 Mar 15;5(5):1326-37
Cyclin-dependent kinases regulate lysosomal degradation of hypoxia-inducible factor 1α to promote cell-cycle progression.
Hubbi ME, Gilkes DM, Hu H, Kshitiz, Ahmed I, Semenza GL
Proceedings of the National Academy of Sciences of the United States of America 2014 Aug 12;111(32):E3325-34
Proceedings of the National Academy of Sciences of the United States of America 2014 Aug 12;111(32):E3325-34
A nontranscriptional role for HIF-1α as a direct inhibitor of DNA replication.
Hubbi ME, Kshitiz, Gilkes DM, Rey S, Wong CC, Luo W, Kim DH, Dang CV, Levchenko A, Semenza GL
Science signaling 2013 Feb 12;6(262):ra10
Science signaling 2013 Feb 12;6(262):ra10
MCM proteins are negative regulators of hypoxia-inducible factor 1.
Hubbi ME, Luo W, Baek JH, Semenza GL
Molecular cell 2011 Jun 10;42(5):700-12
Molecular cell 2011 Jun 10;42(5):700-12
TopBP1 and DNA polymerase-alpha directly recruit the 9-1-1 complex to stalled DNA replication forks.
Yan S, Michael WM
The Journal of cell biology 2009 Mar 23;184(6):793-804
The Journal of cell biology 2009 Mar 23;184(6):793-804
Direct requirement for Xmus101 in ATR-mediated phosphorylation of Claspin bound Chk1 during checkpoint signaling.
Yan S, Lindsay HD, Michael WM
The Journal of cell biology 2006 Apr 24;173(2):181-6
The Journal of cell biology 2006 Apr 24;173(2):181-6
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCM5 Antibody [NB100-79778] - Detection of Human MCM5 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCM5 antibody NB100-79778 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCM5 Antibody [NB100-79778] - Whole cell lysate (3.8, 7.5 or 15 ug for lanes 1, 2 and 3, respectively) from HeLa cells. Antibody used at 0.2 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MCM5 Antibody [NB100-79778] - Human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- MCM5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
