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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- AF1647 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Kremen-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse Kremen-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant human Kremen-2 and recombinant mouse Kremen-2 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references miRNA-431 Prevents Amyloid-β-Induced Synapse Loss in Neuronal Cell Culture Model of Alzheimer's Disease by Silencing Kremen1.
Dickkopf-1 is a master regulator of joint remodeling.
Ross SP, Baker KE, Fisher A, Hoff L, Pak ES, Murashov AK
Frontiers in cellular neuroscience 2018;12:87
Frontiers in cellular neuroscience 2018;12:87
Dickkopf-1 is a master regulator of joint remodeling.
Diarra D, Stolina M, Polzer K, Zwerina J, Ominsky MS, Dwyer D, Korb A, Smolen J, Hoffmann M, Scheinecker C, van der Heide D, Landewe R, Lacey D, Richards WG, Schett G
Nature medicine 2007 Feb;13(2):156-63
Nature medicine 2007 Feb;13(2):156-63
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Mouse Kremen-1 by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Kremen-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1647) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Kremen-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 7.
