PAB1891

antibody from Abnova Corporation

Targeting: KREMEN1 KREMEN, KRM1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB1891

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB1891, RRID:AB_1576728

Product name

KREMEN1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of KREMEN1.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Kremen proteins are Dickkopf receptors that regulate Wnt/beta-catenin signalling.

Mao B, Wu W, Davidson G, Marhold J, Li M, Mechler BM, Delius H, Hoppe D, Stannek P, Walter C, Glinka A, Niehrs C
Nature 2002 Jun 6;417(6889):664-7

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Molecular cloning and characterization of Kremen, a novel kringle-containing transmembrane protein.

Nakamura T, Aoki S, Kitajima K, Takahashi T, Matsumoto K, Nakamura T
Biochimica et biophysica acta 2001 Mar 19;1518(1-2):63-72

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of KREMEN1 (arrow) using KREMEN1 polyclonal antibody (Cat # PAB1891). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KREMEN1 gene (Lane 2) (Origene Technologies).

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with KREMEN1 polyclonal antibody (Cat # PAB1891), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)