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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- PAB2991 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2991, RRID:AB_1576727
- Product name
- KREMEN1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of KREMEN1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Kremen proteins are Dickkopf receptors that regulate Wnt/beta-catenin signalling.
Molecular cloning and characterization of Kremen, a novel kringle-containing transmembrane protein.
Mao B, Wu W, Davidson G, Marhold J, Li M, Mechler BM, Delius H, Hoppe D, Stannek P, Walter C, Glinka A, Niehrs C
Nature 2002 Jun 6;417(6889):664-7
Nature 2002 Jun 6;417(6889):664-7
Molecular cloning and characterization of Kremen, a novel kringle-containing transmembrane protein.
Nakamura T, Aoki S, Kitajima K, Takahashi T, Matsumoto K, Nakamura T
Biochimica et biophysica acta 2001 Mar 19;1518(1-2):63-72
Biochimica et biophysica acta 2001 Mar 19;1518(1-2):63-72
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of KREMEN1 polyclonal antibody (Cat # PAB2991) in A-375 cell line lysates (35 ug/lane).KREMEN1 (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of A-375 cells using KREMEN1 polyclonal antibody (Cat # PAB2991)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
