PA5-119935
antibody from Invitrogen Antibodies
Targeting: ARHGEF18
KIAA0521, MGC15913, P114-RhoGEF, p114RhoGEF
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-119935 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ARHGEF18 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: HepG2 cell lysate, Hela cell lysate, 293 cell lysate, PC-3 cell lysate, Daudi cell lysate, rat kidney tissue, Hela.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of p114RhoGEF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. ARHGEF18 Polyclonal Antibody (Product # PA5-119935) at 1:1,000 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate. Lane 2: Hela cell lysate. Lane 3: 293 cell lysate. Lane 4: PC-3 cell lysate. Lane 5: Daudi cell lysate. Predicted band size: 152 kDa. Observed band size: 114/152 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of p114RhoGEF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. ARHGEF18 Polyclonal Antibody (Product # PA5-119935) at 1:1,000 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: HepG2 cell lysate. Lane 2: Hela cell lysate. Lane 3: 293 cell lysate. Lane 4: PC-3 cell lysate. Lane 5: Daudi cell lysate. Predicted band size: 152 kDa. Observed band size: 114/152 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of paraffin-embedded rat kidney tissue using ARHGEF18 Polyclonal Antibody (Product # PA5-119935). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the ARHGEF18 antibody at a dilution of 1:400 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow Cytometry analysis of ARHGEF18 in HeLa cells using ARHGEF18 Polyclonal Antibody (Product # PA5-119935) at 1 µg/mL (red) compared with Rabbit IgG, monoclonal - Isotype Control (green). After incubation of the primary antibody at 4°C for 1 hour, the cells were stained with a Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1:1,000 dilution for 30 minutes at 4°C (dark incubation). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).