Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA324928 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal KLF5 Antibody (C-term)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal KLF5 Antibody (C-term)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- KLF5
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 2.0 mg/ml
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- KLF5 Antibody (C-term) (Cat. #TA324928) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the KLF5 antibody detected the KLF5 protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of U251 cell stained with KLF5 Antibody (C-term)(Cat#TA324928).U251 cells were incubated with KLF5 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor? 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . KLF5 immunoreactivity is localized to vesicles and Nucleus significantly.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- KLF5 Antibody (C-term) (Cat.#TA324928) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the KLF5 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- KLF5 Antibody (C-term) (Cat. #TA324928) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC
