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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- HPA027368 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA027368, RRID:AB_10602317
- Product name
- Anti-ALKAL1
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
ARVTDKEPKPLLFLPAAGAGRTPSGSRSAEIFPRD
SNLKDKFIKHFTGPVTFSPECSKHF- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
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Supportive validation
- Submitted by
- 57ceb782a6a46
- Main image
- Experimental details
- HEK293 cells were transfected with either empty vector or FAM150A-expressing plasmid. After 24 hours, medium was collected and centrifuged to remove cells. Supernatant was mixed with SDS sample buffer and then loaded into 12,5% SDS-PAGE.
- Sample type
- conditioned medium
- Primary Ab dilution
- 1:5000
- Conjugate
- Unconjugated
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1:5000
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human prostate shows moderate cytoplasmic positivity in glandular cells.
- Sample type
- HUMAN
