620002

antibody from BioLegend

Targeting: RB1CC1 ATG17, Cc1, DRAGOU14, FIP200, KIAA0203, PPP1R131

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

620002

Provider

BioLegend

Proper citation

BioLegend Cat#620002, RRID:AB_2716039

Product name

Purified anti-ATG17

Antibody type

Monoclonal

Reactivity

Human

Host

Rat

Conjugate

Unconjugated

Antibody clone number

W16188A

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Total lysates (15 µg protein) from HeLa (Lane 1), 10nM scrambled siRNA (lane 2), 1nM RB1CC1 siRNA (Lane 3) and 10nM RB1CC1 siRNA treated HeLa cells (Lane 4), 293T (Lane 5), 10nM scrambled siRNA (lane 6), 1nM RB1CC1 siRNA (Lane 7) and 10nM RB1CC1 siRNA treated 293T cells (Lane 8) were resolved by electrophoresis, transferred to nitrocellulose, and probed with 1:500 (1 µg/mL) diluted purified anti-RB1CC1 antibody (clone W16188A). Proteins were visualized by chemiluminescence detection using 1:3000 diluted HRP rat-IgG secondary antibody conjugated to HRP 1:2000 dilution of Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control (lower). Lane M: Molecular weight ladder.

Conjugate

Unconjugated