12872-1-AP

antibody from Invitrogen Antibodies

Targeting: ALG1 CDG1K, HMAT1, HMT-1, Mat-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12872-1-AP

Provider

Invitrogen Antibodies

Product name

ALG1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: HNYGYSIMG LVHGPNHPLV LLAKWYEKFF GRLSHLNLCV TNAMREDLAD NWHIRAVTVY DKPASFFKET PLDLQHRLFM KLGSMHSPFR ARSEPEDPVT ERSAFTERDA GSGLVTRLRE RPALLVSSTS WTEDEDFSIL LAALEKFEQL TLDGHNLPSL VCVITGKGPM REYYSRLIHQ KHFQHIQVCT PWLEAEDYPL LLGSADLGVC LHTSSSGLDL PMKVVDMFGC CLPVCAVNFK CLHELVKHEE NGLVFEDSEE LAAQLQMLFS NFPDPAGKLN QFRKNLRESQ QLRWDESWVR TVLPLVMDT (157-464 aa encoded by BC031095)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.33 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 12872-1-AP (ALG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 12872-1-AP (ALG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 12872-1-AP ( ALG1 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 12872-1-AP ( ALG1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-ALG1 (IP:12872-1-AP, 4ug; Detection:12872-1-AP 1:500) with HeLa cells lysate 3080 ug.