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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- MAB1097 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB1097, RRID:AB_1581177
- Product name
- VAMP2 monoclonal antibody, clone 3E5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant VAMP2.
- Isotype
- IgG
- Antibody clone number
- 3E5
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Synaptophysin I controls the targeting of VAMP2/synaptobrevin II to synaptic vesicles.
Amisyn, a novel syntaxin-binding protein that may regulate SNARE complex assembly.
VAMP-1 and VAMP-2 gene expression in rat spinal motoneurones: differential regulation after neuronal injury.
Pennuto M, Bonanomi D, Benfenati F, Valtorta F
Molecular biology of the cell 2003 Dec;14(12):4909-19
Molecular biology of the cell 2003 Dec;14(12):4909-19
Amisyn, a novel syntaxin-binding protein that may regulate SNARE complex assembly.
Scales SJ, Hesser BA, Masuda ES, Scheller RH
The Journal of biological chemistry 2002 Aug 2;277(31):28271-9
The Journal of biological chemistry 2002 Aug 2;277(31):28271-9
VAMP-1 and VAMP-2 gene expression in rat spinal motoneurones: differential regulation after neuronal injury.
Jacobsson G, Piehl F, Meister B
The European journal of neuroscience 1998 Jan;10(1):301-16
The European journal of neuroscience 1998 Jan;10(1):301-16
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- Experimental details
- Western blot analysis of recombinant Synaptobrevin 1 , 2 (each 20 ng) and rat brain extracts (20 ug). Lane 1 : Synaptobrevin 1. Lane 2 : Synaptobrevin 2. Lane 3 : Rat brain. To probed with VAMP2 monoclonal antibody , clone 3E5 (1 : 2000) (Cat # MAB1097). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
