Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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Validation data
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- Product number
- H00005165-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005165-M01, RRID:AB_546922
- Product name
- Mouse Monoclonal PDK3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PDK3 - pyruvate dehydrogenase kinase, isozyme 3
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Additive effects of clofibric acid and pyruvate dehydrogenase kinase isoenzyme 4 (PDK4) deficiency on hepatic steatosis in mice fed a high saturated fat diet.
Control of oxidative phosphorylation by vitamin A illuminates a fundamental role in mitochondrial energy homoeostasis.
Induction of pyruvate dehydrogenase kinase-3 by hypoxia-inducible factor-1 promotes metabolic switch and drug resistance.
Pyruvate dehydrogenase kinase-4 deficiency lowers blood glucose and improves glucose tolerance in diet-induced obese mice.
Hwang B, Wu P, Harris RA
The FEBS journal 2012 May;279(10):1883-93
The FEBS journal 2012 May;279(10):1883-93
Control of oxidative phosphorylation by vitamin A illuminates a fundamental role in mitochondrial energy homoeostasis.
Acin-Perez R, Hoyos B, Zhao F, Vinogradov V, Fischman DA, Harris RA, Leitges M, Wongsiriroj N, Blaner WS, Manfredi G, Hammerling U
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Feb;24(2):627-36
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Feb;24(2):627-36
Induction of pyruvate dehydrogenase kinase-3 by hypoxia-inducible factor-1 promotes metabolic switch and drug resistance.
Lu CW, Lin SC, Chen KF, Lai YY, Tsai SJ
The Journal of biological chemistry 2008 Oct 17;283(42):28106-14
The Journal of biological chemistry 2008 Oct 17;283(42):28106-14
Pyruvate dehydrogenase kinase-4 deficiency lowers blood glucose and improves glucose tolerance in diet-induced obese mice.
Jeoung NH, Harris RA
American journal of physiology. Endocrinology and metabolism 2008 Jul;295(1):E46-54
American journal of physiology. Endocrinology and metabolism 2008 Jul;295(1):E46-54
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PDK3 Antibody (2B11) [H00005165-M01] - Analysis of PDK3 expression in transfected 293T cell line by PDK3 monoclonal antibody (M01), clone 2B11.Lane 1: PDK3 transfected lysate(46.9 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PDK3 Antibody (2B11) [H00005165-M01] - PDK3 monoclonal antibody (M01), clone 2B11 Analysis of PDK3 expression in MCF-7.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: PDK3 Antibody (2B11) [H00005165-M01] - Detection limit for recombinant GST tagged PDK3 is approximately 0.03ng/ml as a capture antibody.