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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [4]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- 12215-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PDK3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: GVIEYKEKF GFDPFISTNI QYFLDRFYTN RISFRMLINQ HTLLFGGDTN PVHPKHIGSI DPTCNVADVV KDAYETAKML CEQYYLVAPE LEVEEFNAKA PDKPIQVVYV PSHLFHMLFE LFKNSMRATV ELYEDRKEGY PAVKTLVTLG KEDLSIKISD LGGGVPLRKI DRLFNYMYST APRPSLEPTR AAPLAGFGYG LPISRLYARY FQGDLKLYSM EGVGTDAVIY LKALSSESFE RL (129-369 aa encoded by BC015948)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.35 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12215-1-AP (PDK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse heart tissue were subjected to SDS PAGE followed by western blot with 12215-1-AP (PDK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse heart tissue were subjected to SDS PAGE followed by western blot with 12215-1-AP (PDK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse heart tissue were subjected to SDS PAGE followed by western blot with 12215-1-AP (PDK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skeletal muscle slide using 12215-1-AP (PDK3 Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skeletal muscle slide using 12215-1-AP (PDK3 Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart slide using 12215-1-AP ( PDK3 Antibody) at dilution of 1:50.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart slide using 12215-1-AP ( PDK3 Antibody) at dilution of 1:50.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-PDK3 (IP:12215-1-AP, 4ug; Detection:12215-1-AP 1:1000) with mouse heart tissue lysate 4000ug.
