Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-76386 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal TRAPPC5 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 2 - 8 C 1 year from date of receipt
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRAPPC5 Antibody [NBP2-76386] - Samples: Whole cell lysate (50 ug) from HEK293T, Jurkat, K-562, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRAPPC5 antibody NBP2-76386 used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: TRAPPC5 Antibody [NBP2-76386] - Detection of human TRAPPC5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRAPPC5 antibody NBP2-76386 used for IP at 100 ul per reaction. TRAPPC5 was also immunoprecipitated by another rabbit anti-TRAPPC5 antibody. For blotting immunoprecipitated TRAPPC5, NBP2-76386 was used at 1:400. Detection: Chemiluminescence with an exposure time of 30 seconds.
