A305-807A-T

antibody from Invitrogen Antibodies

Targeting: TRAPPC5 MGC52424, TRS31

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A305-807A-T

Provider

Invitrogen Antibodies

Product name

TRAPPC5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human and mouse TRAPPC5 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, Jurkat, K-562, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRAPPC5 antibody A305-807A-M (lot A305-807A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human TRAPPC5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRAPPC5 antibody (Product # A305-807A-T; lot A305-807A-T-1) used for IP at 100 µL per reaction. TRAPPC5 was also immunoprecipitated by rabbit anti-TRAPPC5 antibody BL23481. For blotting immunoprecipitated TRAPPC5, Product # A305-807A-T was used at 1:400. Detection: Chemiluminescence with an exposure time of 30 seconds.