12083-2-AP

antibody from Invitrogen Antibodies

Targeting: CD164 DFNA66, MGC-24, MUC-24

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

12083-2-AP

Provider

Invitrogen Antibodies

Product name

CD164 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: DKNTTQHPN VTTLAPISNV TSAPVTSLPL VTTPAPETCE GRNSCVSCFN VSVVNTTCFW IECKDESYCS HNSTVSDCQV GNTTDFCSVS TATPVPTANS TAKPTVQPSP STTSKTVTTS GTTNNTVTPT SQPVRKSTFD AASFIGGIVL VLGVQAVIFF LYKFCKSKER NYHTL (24-197 aa encoded by B C011522)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.39 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 12083-2-AP (CD164 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas cancer using 12083-2-AP (CD164 antibody) at dilution of 1:50 (under 10x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 K-562 cells were intracellularly stained with 0.5 µg Anti-Human CD164 (Product # 12083-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (green), and 0.5 µg Control Antibody. Cells were fixed with 90% MeOH .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 K-562 cells were stained with 0.2ug CD164 antibody (12083-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.