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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AF5638 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse SLAIN1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse SLAIN1.
- Reactivity
- Human, Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q68FF7- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human/Mouse SLAIN1 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, human cerebellum tissue, and BG01V human embryonic stem cells. PVDF membrane was probed with 1 µg/mL of Human/Mouse SLAIN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5638) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for SLAIN1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- SLAIN1 in D3 Mouse Cell Line. SLAIN1 was detected in immersion fixed D3 mouse embryonic stem cell line using 10 µg/mL Human/Mouse SLAIN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5638) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
