NBP1-18931

antibody from Novus Biologicals

Targeting: MYH10 NMMHCB

Western blot (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-18931

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-18931, RRID:AB_1625795

Product name

Rabbit Polyclonal non-muscle heavy chain 10 Myosin Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: non-muscle heavy chain 10 Myosin Antibody [NBP1-18931] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-Myosin-10 antibody used for IP at 3 mcg/mg lysate. Myosin-10 was also immunoprecipitated by rabbit anti-Myosin-10 antibody NBP1-18931, which recognizes a downstream epitope.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: non-muscle heavy chain 10 Myosin Antibody [NBP1-18931] - Detection of human Myosin-10 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-Myosin-10 antibody NBP1-18931 used for IP at 3 ug/mg lysate. Myosin-10 was also immunoprecipitated by rabbit anti-Myosin-10 antibody NBP1-18932, which recognizes a downstream epitope. For blotting immunoprecipitated Myosin-10, NBP1-18931 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.