H00005457-M04

antibody from Abnova Corporation

Targeting: POU4F1 BRN3A, RDC-1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005457-M04

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005457-M04, RRID:AB_10646042

Product name

POU4F1 monoclonal antibody (M04), clone 7B4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant POU4F1.

Antigen sequence

QAWLEEAEGAQREKMNKPELFNGGEKKRKRTSIAA
PEKRSLEAYFAVQPRPSSEKIAAIAEKLDLKKNVV
RVWFCNQRQKQKRMKFSATY


Isotype

IgG

Antibody clone number

7B4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of POU4F1 expression in transfected 293T cell line by POU4F1 monoclonal antibody (M04), clone 7B4.Lane 1: POU4F1 transfected lysate(46.09 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged POU4F1 is 3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol