MA5-25428

antibody from Invitrogen Antibodies

Targeting: ACOT12 Cach, STARD15, THEAL

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-25428

Provider

Invitrogen Antibodies

Product name

ACOT12 Monoclonal Antibody (OTI1B9)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI1B9

Vial size

100 µL

Concentration

0.91 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Exosomal miR-155-5p derived from glioma stem-like cells promotes mesenchymal transition via targeting ACOT12.

Bao Z, Zhang N, Niu W, Mu M, Zhang X, Hu S, Niu C
Cell death & disease 2022 Aug 19;13(8):725

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ACOT12 in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with ACOT12 (Product # MA5-25428) monoclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded human prostate tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a ACOT12 monoclonal antibody (Product # MA5-25428).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded human kidney tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a ACOT12 monoclonal antibody (Product # MA5-25428).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded human liver tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a ACOT12 monoclonal antibody (Product # MA5-25428).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded human lymph node tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a ACOT12 monoclonal antibody (Product # MA5-25428).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded carcinoma of human prostate tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a ACOT12 monoclonal antibody (Product # MA5-25428).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded adenocarcinoma of human colon tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a ACOT12 monoclonal antibody (Product # MA5-25428).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry was performed on paraffin-embedded human lymphoma tissue. To expose target proteins, 10mM citric buffer, pH6.0, 100°C for 10min was used. Following antigen retrieval, tissues were probed with a ACOT12 monoclonal antibody (Product # MA5-25428).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 5 ACOT12 is the downstream target of miR-155-5p and inhibits glioma development. A Prediction of downstream target genes of miR-155-5p via four online databases (miRDB, TargetScan, miRTarBase); B Sequence alignment showing the potential binding sites between ACOT12 mRNA and miR-155-5p; Luciferase reporter activity of WT or MUT ACOT12 in 293 T cells co-transfected with miR-155-5p mimic; C , D Relative mRNA and protein levels of ACOT12 in glioma cells transfected with miR-155-5p inhibitor of mimic; E , F Relative expression level of ACOT12 in glioma cells transfected with si-ACOT12 alone or co-transfected with si-ACOT12 and miR-155-5p inhibitor detected by RT-qPCR and western blotting; G The Pearson correlation between miR-155-5p level and ACOT12 level was measured in glioma tissues. The DeltaCt values (normalized to GAPDH) were subjected to Pearson correlation analysis ( R = -0.43, P < 0.005); H Relative expression level of ACOT12 in glioma sections of different grades detected by IHC; I , J Migration and invasion of glioma cells transfected with miR-155-5p inhibitor or co-transfected with ACOT12 siRNA and miR-155-5p inhibitor, as detected by wound healing assays and Matrigel-coated Transwell assays. Representative images are shown. Data represent as the mean +- SEM from three independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001 (Student's t- test).