A305-622A-T

antibody from Invitrogen Antibodies

Targeting: FRAS1 FLJ14927, FLJ22031, KIAA1500

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A305-622A-T

Provider

Invitrogen Antibodies

Product name

FRAS1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

The recommended shelf life for this product is 1 year from date of receipt.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human FRAS1 by western blot. Samples: Whole cell lysate (50 µg) from Hep-G2, RKO, Jurkat, K-562, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FRAS1 antibody (Product # A305-622A-T lot 2) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human FRAS1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FRAS1 antibody A305-622A-M (lot A305-622A-M-1) used for IP at 20 µl per reaction. FRAS1 was also immunoprecipitated by rabbit anti-FRAS1 antibody BL22247. For blotting immunoprecipitated FRAS1, A305-622A-M was used at 1:100. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human FRAS1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-FRAS1 antibody A305-622A-M (lot A305-622A-M-1) used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human FRAS1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-FRAS1 antibody (Product # A305-622A-T; lot 2) used for IP at 6 µg per reaction. FRAS1 was also immunoprecipitated by a previous lot of this antibody (Product # A305-622A-T; lot 1). For blotting immunoprecipitated FRAS1, Product # A305-622A-T-1 was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.