Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-24797 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ADFP Monoclonal Antibody (2C5A3)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2C5A3
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Differential Effects of Oleic and Palmitic Acids on Lipid Droplet-Mitochondria Interaction in the Hepatic Cell Line HepG2.
Eynaudi A, Díaz-Castro F, Bórquez JC, Bravo-Sagua R, Parra V, Troncoso R
Frontiers in nutrition 2021;8:775382
Frontiers in nutrition 2021;8:775382
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Perilipin-2/ADFP in ADFP cells. The membrane was probed with Perilipin-2/ADFP monoclonal antibody (Product # MA5-24797).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ADFP in (1) HEK293 and (2) PLIN2 hIgGFc transfected HEK293 cell lysate. Samples were incubated in ADFP monoclonal antibody (Product # MA5-24797).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ADFP in human ADFP recombinant protein. Samples were incubated in ADFP monoclonal antibody (Product # MA5-24797). Expected MW is 42.6 kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ADFP in (1) HEK293 and (2) PLIN2 hIgGFc transfected HEK293 cell lysate. Samples were incubated in ADFP monoclonal antibody (Product # MA5-24797).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ADFP in human ADFP recombinant protein. Samples were incubated in ADFP monoclonal antibody (Product # MA5-24797). Expected MW is 42.6 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Perilipin-2/ADFP in HepG2 cells using Perilipin-2/ADFP monoclonal antibody (Product # MA5-24797). Cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The sample was then incubated with anti-ADFP/Perilipin-2 monoclonal antibody (Product # MA5-24797) and detected with anti-mouse Dylight 488 (1:500) and Phalloidin (1:200). Images were taken at 40x magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ADFP in paraffin-embedded human esophageal cancer tissues. Samples were incubated in ADFP monoclonal antibody (Product # MA5-24797). DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ADFP in paraffin-embedded human liver cancer tissues. Samples were incubated in ADFP monoclonal antibody (Product # MA5-24797). DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Oleic and palmitic acids induce different patterns of lipid droplet-mitochondria tether proteins in HepG2 cells. (A) Representative Western blots of the proteins associated with lipid droplet (LD)-mitochondria physical interaction: Perilipin 2 and 5 (PLIN2, PLIN5), and Mitofusin 2 (MFN2) in HepG2 cells treated with 200 muM of oleic acid (OA) or palmitic acid (PA) for 0-24 h. GAPDH was used as a loading control. (B-D) Densitometric quantification of the proteins indicated in A . Data are expressed as mean +- SEM of N = 7 for PLIN2 and N = 4 for PLIN5 and MFN2. * p < 0.05; ** p < 0.01 vs. BSA; # p < 0.05; and ## p < 0.01 vs. OA at the same time conditions.
