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- Antibody Data
- Antigen structure
- References [13]
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- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB392-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CXCL9/MIG Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CXCL9/MIG in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant mouse (rm) CXCL9, recombinant human CXCL10.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q07325- Isotype
- IgG
- Antibody clone number
- 49106
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references CXCL9 and CXCL10 predict survival and are regulated by cyclooxygenase inhibition in advanced serous ovarian cancer.
Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation.
Evidence for a pathophysiological role of keratinocyte-derived type III interferon (IFNλ) in cutaneous lupus erythematosus.
Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
Expression of CXCL9, -10, -11, and CXCR3 in the tear film and ocular surface of patients with dry eye syndrome.
CXCR3 ligands are augmented during the pathogenesis of pulmonary sarcoidosis.
Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients.
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
Up-regulation of the interferon gamma (IFN-gamma)-inducible chemokines IFN-inducible T-cell alpha chemoattractant and monokine induced by IFN-gamma and of their receptor CXC receptor 3 in human renal cell carcinoma.
Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
Bronger H, Singer J, Windmüller C, Reuning U, Zech D, Delbridge C, Dorn J, Kiechle M, Schmalfeldt B, Schmitt M, Avril S
British journal of cancer 2016 Aug 23;115(5):553-63
British journal of cancer 2016 Aug 23;115(5):553-63
Intracellular coexpression of CXC- and CC- chemokine receptors and their ligands in human melanoma cell lines and dynamic variations after xenotransplantation.
Pinto S, Martínez-Romero A, O'Connor JE, Gil-Benso R, San-Miguel T, Terrádez L, Monteagudo C, Callaghan RC
BMC cancer 2014 Feb 22;14:118
BMC cancer 2014 Feb 22;14:118
Evidence for a pathophysiological role of keratinocyte-derived type III interferon (IFNλ) in cutaneous lupus erythematosus.
Zahn S, Rehkämper C, Kümmerer BM, Ferring-Schmidt S, Bieber T, Tüting T, Wenzel J
The Journal of investigative dermatology 2011 Jan;131(1):133-40
The Journal of investigative dermatology 2011 Jan;131(1):133-40
Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 15;184(6):2886-98
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 15;184(6):2886-98
Expression of CXCL9, -10, -11, and CXCR3 in the tear film and ocular surface of patients with dry eye syndrome.
Yoon KC, Park CS, You IC, Choi HJ, Lee KH, Im SK, Park HY, Pflugfelder SC
Investigative ophthalmology & visual science 2010 Feb;51(2):643-50
Investigative ophthalmology & visual science 2010 Feb;51(2):643-50
CXCR3 ligands are augmented during the pathogenesis of pulmonary sarcoidosis.
Busuttil A, Weigt SS, Keane MP, Xue YY, Palchevskiy V, Burdick MD, Huang C, Zisman DA, Fishbein M, Lynch JP 3rd, Strieter RM, Elashoff RM, Belperio JA
The European respiratory journal 2009 Sep;34(3):676-86
The European respiratory journal 2009 Sep;34(3):676-86
Intratumoral induction of CD103 triggers tumor-specific CTL function and CCR5-dependent T-cell retention.
Franciszkiewicz K, Le Floc'h A, Jalil A, Vigant F, Robert T, Vergnon I, Mackiewicz A, Benihoud K, Validire P, Chouaib S, Combadière C, Mami-Chouaib F
Cancer research 2009 Aug 1;69(15):6249-55
Cancer research 2009 Aug 1;69(15):6249-55
Upregulation of human cytomegalovirus by HIV type 1 in human lymphoid tissue ex vivo.
Biancotto A, Iglehart SJ, Lisco A, Vanpouille C, Grivel JC, Lurain NS, Reichelderfer PS, Margolis LB
AIDS research and human retroviruses 2008 Mar;24(3):453-62
AIDS research and human retroviruses 2008 Mar;24(3):453-62
Down-modulation of CXCR3 surface expression and function in CD8+ T cells from cutaneous T cell lymphoma patients.
Winter D, Moser J, Kriehuber E, Wiesner C, Knobler R, Trautinger F, Bombosi P, Stingl G, Petzelbauer P, Rot A, Maurer D
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):4272-82
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):4272-82
Abnormal activation and cytokine spectra in lymph nodes of people chronically infected with HIV-1.
Biancotto A, Grivel JC, Iglehart SJ, Vanpouille C, Lisco A, Sieg SF, Debernardo R, Garate K, Rodriguez B, Margolis LB, Lederman MM
Blood 2007 May 15;109(10):4272-9
Blood 2007 May 15;109(10):4272-9
HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1.
Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P
AIDS (London, England) 2007 Jun 19;21(10):1263-72
AIDS (London, England) 2007 Jun 19;21(10):1263-72
Up-regulation of the interferon gamma (IFN-gamma)-inducible chemokines IFN-inducible T-cell alpha chemoattractant and monokine induced by IFN-gamma and of their receptor CXC receptor 3 in human renal cell carcinoma.
Suyama T, Furuya M, Nishiyama M, Kasuya Y, Kimura S, Ichikawa T, Ueda T, Nikaido T, Ito H, Ishikura H
Cancer 2005 Jan 15;103(2):258-67
Cancer 2005 Jan 15;103(2):258-67
Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
Kohrgruber N, Gröger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
Journal of immunology (Baltimore, Md. : 1950) 2004 Dec 1;173(11):6592-602
Journal of immunology (Baltimore, Md. : 1950) 2004 Dec 1;173(11):6592-602
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CXCL9/MIG in THP-1 Human Cell Line. CXCL9/MIG was detected in immersion fixed THP-1 human acute monocytic leukemia cell line stimulated with IFN-gamma for 24 hours using Mouse Anti-Human CXCL9/MIG Monoclonal Antibody (Catalog # MAB392) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CXCL9/MIG and Neutralization by Human CXCL9/MIG Antibody. Recombinant Human CXCL9/MIG (Catalog # 392-MG) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL9/MIG (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCL9/MIG Monoclonal Antibody (Catalog # MAB392). The ND50 is typically 4-20 µg/mL.
