GTX22922

antibody from GeneTex

Targeting: RASA1 CM-AVM, GAP, p120, p120GAP, p120RASGAP, RASA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

GTX22922

Provider

GeneTex

Proper citation

GeneTex Cat#GTX22922, RRID:AB_371449

Product name

RASA1 antibody [B4F8]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat, Bovine, Simian

Host

Mouse

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis of GAP in 25 ug of HeLa, A431 and mouse brain lysates. Proteins were transferred to a PVDF membrane and blocked with a blocking buffer at 4?C overnight. The membrane was probed with GAP antibody [B4F8] at a dilution of 1:200 overnight at 4¢XC, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescent analysis of GAP (green) in HeLa cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with GAP antibody [B4F8] in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4?C in a humidified chamber. Cells were washed with PBST and incubated with a proper secondary antibody. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with DAPI for 5-10 minutes in the dark. Images were taken at a magnification of 60x.