Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 16232-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NUP54 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAFNFGAPS GTSGTAAATA APAGGFGGFG TTSTTAGSAF SFSAPTNTGT TGLFGGTQNK GFGFGTGFGT TTGTSTGLGT GLGTGLGFGG FNTQQQQQTT LGGLFSQPTQ APTQSNQLIN TASALSAPTL LGDERDAILA KWNQLQAFWG TGKGYFNNNI PPVEFTQENP FCRFKAVGYS CMPSNKDEDG LVVLVFNKKE TEIRSQQQQL VESLHKVLGG NQTLTVNVEG TKTLPDDQTE VVIYVVERSP NGTSRRVPAT TLYAHFEQAN IKTQLQQLGV TLSMTRTELS PAQIKQLLQN PPAGVDPIIW EQAKVDNPDS EKLIPVPMVG FKELLRRLKV QDQMTKQHQT RL (1-351 aa encoded by BC012559)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.2 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse heart tissue were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse liver tissue were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A549 cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 16232-1-AP (NUP54 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA) fixed HepG2 cells using 16232-1-AP (NUP54 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).