PA5-32030

antibody from Invitrogen Antibodies

Targeting: ASGR1 CLEC4H1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-32030

Provider

Invitrogen Antibodies

Product name

ASGR1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: Huh-7, HepG2, mouse liver.

Concentration

0.3 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ASGPR1 using 30 µg of HepG2 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with an ASGPR1 polyclonal antibody (Product # PA5-32030) at a dilution of 1:3000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ASGPR1 using 30 µg of NIH-3T3 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with an ASGPR1 polyclonal antibody (Product # PA5-32030) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ASGR1 was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ASGR1 Polyclonal Antibody (Product # PA5-32030) at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ASGR1 Polyclonal Antibody (Product # PA5-32030). Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with ASGR1 Polyclonal Antibody (Product # PA5-32030) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ASGR1 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ASGR1 Polyclonal Antibody (Product # PA5-32030) at a dilution of 1:3000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of ASGR1 was performed in HepG2 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ASGR1 Polyclonal Antibody (Product # PA5-32030) diluted at 1:500. Blue: Hoechst 33342 staining.