Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
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- Product number
- PA5-32030 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ASGR1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Huh-7, HepG2, mouse liver. Predicted reactivity: Rat (80%), Pig (84%), Bovine (82%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ASGPR1 using 30 µg of HepG2 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with an ASGPR1 polyclonal antibody (Product # PA5-32030) at a dilution of 1:3000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ASGPR1 using 30 µg of NIH-3T3 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with an ASGPR1 polyclonal antibody (Product # PA5-32030) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ASGR1 was performed by separating 50 µg of mouse tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ASGR1 Polyclonal Antibody (Product # PA5-32030) at a dilution of 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ASGR1 Polyclonal Antibody (Product # PA5-32030). Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with ASGR1 Polyclonal Antibody (Product # PA5-32030) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ASGR1 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ASGR1 Polyclonal Antibody (Product # PA5-32030) at a dilution of 1:3000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ASGR1 was performed in HepG2 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ASGR1 Polyclonal Antibody (Product # PA5-32030) diluted at 1:500. Blue: Hoechst 33342 staining.