PA5-35865

antibody from Invitrogen Antibodies

Targeting: CETN3 CEN3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-35865

Provider

Invitrogen Antibodies

Product name

Centrin 3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A549, H1299, HCT-116, Neuro2A, CD830, NIH-3T3, Raw264.7, C2C12, PC-12, Rat-2.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Centrin-3 using Various whole cell extracts (30 µg). Samples were loaded onto a 15% SDS-PAGE gel and probed with a Centrin-3 polyclonal antibody (Product # PA5-35865) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Centrin3 antibody detects Centrin3 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Centrin3 antibody Centrin 3 Polyclonal Antibody (Product # PA5-35865) diluted by 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Centrin3 antibody detects Centrin3 protein by western blot analysis. Various whole cell extracts of mouse and rat (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with Centrin3 antibody Centrin 3 Polyclonal Antibody (Product # PA5-35865) diluted by 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Centrin3 antibody detects Centrin3 protein at centrosome by immunofluorescent analysis. Sample: A549 cells were fixed in 100% MeOH for 5 min. Green: Centrin3 protein stained by Centrin3 antibody (Product # PA5-35865) diluted at 1:1,000. Red: alpha Tubulin Polyclonal Antibody [B-5-1-2] (Product # MA1-25054) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Centrin3 antibody detects Centrin3 protein at cytosol on mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. Centrin3 antibody (Product # PA5-35865) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.